Review



bam h i  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    New England Biolabs bam h i
    Bam H I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+h+i/BamHI-HF/pmc12216599-58-0-4
    Average 99 stars, based on 1832 article reviews
    bam h i - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Marker:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Over Expression:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Plasmid Preparation:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Cloning:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Homologous Recombination:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Sequencing:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Purification:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Recombinant:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Expressing:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Generated:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.

    Ligation:

    Article Title: Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription
    Article Snippet: Recombinant plasmids expressing HBDSP genes (GenBank No. FJ151414.1 ), phouge-HBDSP, were generated by ligation of HBDSP into the Xba I and Bam H I (New England BioLabs) sites of the pCDH-EF1-MCS-T2A-Puro vector (System Biosciences, USA, abbreviated as phouge in this manuscript), and pDsRed-HBDSP were obtained by ligation of HBDSP into the Xho I and Sal I (New England BioLabs) sites of pDsRed-monomer-hyg-N1 vector (Clontech, USA, abbreviated as pDsRed).

    Article Title: Genome-Wide Identification of GmARF9b / GmARF2a Negatively Regulate Root Growth in Soybean
    Article Snippet: The fragments were then cloned into the pFGC5941-35S-intron-(GFP-Bar marker) overexpression vector using the Clon Express II One Step Cloning Kit with homologous recombination enzyme (Vazyme, Nanjing, China), utilizing the restriction sites Asc I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, Ipswich, MA, USA).

    Article Title: Isolation and Characterization of a Novel Virulent Phage ASG01 of Aeromonas salmonicida and Its Cell Wall Hydrolase Activity
    Article Snippet: The sequence and pET28a plasmid were digested with Sal I (New England Biolabs, Ipswich, MA, USA) and Bam H I (New England Biolabs, MA, USA) at 37 °C for 4 h, and subsequently the gel was purified.


    Article Title: GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
    Article Snippet: GST-Snail and its truncated plasmids were subcloned into pGEX-4T-1(28-9545-49, GE Healthcare) by PCR between Bam H I (R3136M, NEB) and Eco R I (R3101M, NEB) restriction enzyme sites.

    Article Title: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells
    Article Snippet: Bam H I (R013S), Kpn I (R0142S), Nde I (R0111S), Hin d III (R0104S), Xba I (R0145S), T4 DNA Ligase (M0202 L) and DNA Polymerase I, Large (Klenow) Fragment (M0210S) were procured from New England Biolabs (NEB), USA. dNTP mix (U1515) was purchased from Promega, USA.



    Similar Products

    bam h i  (TaKaRa)
    99
    TaKaRa bam h i
    Bam H I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+h+i/BamH+I/pmc13087886-104-11-14
    Average 99 stars, based on 1 article reviews
    bam h i - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    bam h i hin d iii sites  (TaKaRa)
    99
    TaKaRa bam h i hin d iii sites
    Bam H I Hin D Iii Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+h+i/Hind+III/pmc12628632-82-11-24
    Average 99 stars, based on 1 article reviews
    bam h i hin d iii sites - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    bam h  (TaKaRa)
    99
    TaKaRa bam h
    Bam H, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+h+i/BamH+I/bio_rxiv__2025__09__13__674623-177-16-26
    Average 99 stars, based on 1 article reviews
    bam h - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    bam h i digested 16318h gfp vector  (TaKaRa)
    99
    TaKaRa bam h i digested 16318h gfp vector
    Structure and localization analysis of TaSWEET13h transporter. (A) TaSWEET13h protein sequence. Blue boxes and orange dots represent the transmembrane domain and MtN3_slv domains, respectively. (B) TMHMM analysis of the transmembrane domain profile of TaSWEET13h. (C) Cartoon image of the 3D structure of TaSWEET13h resolved by AlphaFold 2. (D) Subcellular localization of TaSWEET13h in wheat leaf protoplasts. The protoplasts transfected with the <t>16318h-</t> GFP plasmid are used as a control, with mCherry fluorescence representing the marker of cytoplasmic membrane. Scale bar=5 μm.
    Bam H I Digested 16318h Gfp Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+h+i/BamH+I/pmc12237909-90-7-13
    Average 99 stars, based on 1 article reviews
    bam h i digested 16318h gfp vector - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    quickcut bam h  (TaKaRa)
    99
    TaKaRa quickcut bam h
    Structure and localization analysis of TaSWEET13h transporter. (A) TaSWEET13h protein sequence. Blue boxes and orange dots represent the transmembrane domain and MtN3_slv domains, respectively. (B) TMHMM analysis of the transmembrane domain profile of TaSWEET13h. (C) Cartoon image of the 3D structure of TaSWEET13h resolved by AlphaFold 2. (D) Subcellular localization of TaSWEET13h in wheat leaf protoplasts. The protoplasts transfected with the <t>16318h-</t> GFP plasmid are used as a control, with mCherry fluorescence representing the marker of cytoplasmic membrane. Scale bar=5 μm.
    Quickcut Bam H, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+h+i/BamH+I/pmc12286206-53-0-7
    Average 99 stars, based on 1 article reviews
    quickcut bam h - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    bam h i  (New England Biolabs)
    99
    New England Biolabs bam h i
    Structure and localization analysis of TaSWEET13h transporter. (A) TaSWEET13h protein sequence. Blue boxes and orange dots represent the transmembrane domain and MtN3_slv domains, respectively. (B) TMHMM analysis of the transmembrane domain profile of TaSWEET13h. (C) Cartoon image of the 3D structure of TaSWEET13h resolved by AlphaFold 2. (D) Subcellular localization of TaSWEET13h in wheat leaf protoplasts. The protoplasts transfected with the <t>16318h-</t> GFP plasmid are used as a control, with mCherry fluorescence representing the marker of cytoplasmic membrane. Scale bar=5 μm.
    Bam H I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+h+i/BamHI-HF/pmc12216599-58-0-4
    Average 99 stars, based on 1 article reviews
    bam h i - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    quickcut bam h i  (TaKaRa)
    99
    TaKaRa quickcut bam h i
    Structure and localization analysis of TaSWEET13h transporter. (A) TaSWEET13h protein sequence. Blue boxes and orange dots represent the transmembrane domain and MtN3_slv domains, respectively. (B) TMHMM analysis of the transmembrane domain profile of TaSWEET13h. (C) Cartoon image of the 3D structure of TaSWEET13h resolved by AlphaFold 2. (D) Subcellular localization of TaSWEET13h in wheat leaf protoplasts. The protoplasts transfected with the <t>16318h-</t> GFP plasmid are used as a control, with mCherry fluorescence representing the marker of cytoplasmic membrane. Scale bar=5 μm.
    Quickcut Bam H I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bam+h+i/BamH+I/pmc11846821-242-28-32
    Average 99 stars, based on 1 article reviews
    quickcut bam h i - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Structure and localization analysis of TaSWEET13h transporter. (A) TaSWEET13h protein sequence. Blue boxes and orange dots represent the transmembrane domain and MtN3_slv domains, respectively. (B) TMHMM analysis of the transmembrane domain profile of TaSWEET13h. (C) Cartoon image of the 3D structure of TaSWEET13h resolved by AlphaFold 2. (D) Subcellular localization of TaSWEET13h in wheat leaf protoplasts. The protoplasts transfected with the 16318h- GFP plasmid are used as a control, with mCherry fluorescence representing the marker of cytoplasmic membrane. Scale bar=5 μm.

    Journal: Frontiers in Plant Science

    Article Title: Wheat endosperm-specific transcription factor TaDOF6 enhances grain development by regulating TaSWEET13h expression and facilitating sugar and gibberellin transport

    doi: 10.3389/fpls.2025.1608090

    Figure Lengend Snippet: Structure and localization analysis of TaSWEET13h transporter. (A) TaSWEET13h protein sequence. Blue boxes and orange dots represent the transmembrane domain and MtN3_slv domains, respectively. (B) TMHMM analysis of the transmembrane domain profile of TaSWEET13h. (C) Cartoon image of the 3D structure of TaSWEET13h resolved by AlphaFold 2. (D) Subcellular localization of TaSWEET13h in wheat leaf protoplasts. The protoplasts transfected with the 16318h- GFP plasmid are used as a control, with mCherry fluorescence representing the marker of cytoplasmic membrane. Scale bar=5 μm.

    Article Snippet: The TaDOF6 CDS was cloned into the Bam H I-digested 16318h- GFP vector (TaKaRa), provided by Dr. Yimiao Tang (Beijing Academy of Agriculture and Forestry Sciences).

    Techniques: Sequencing, Transfection, Plasmid Preparation, Control, Fluorescence, Marker, Membrane